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Through this ICMM space slack account you will be able to navigate the funk, padel, soccer, dance, articles-icmm, scientic-questions channel and many other things such as helping the new icmm-nautas. You can also open your own #channel to find people who share your interests. Join us! Read more

About

The Materials Science Institute of Madrid (ICMM) is a research centre of the Spanish National Research Council (CSIC) that belongs to the area Materia.

 

Our mission is to generate new fundamental and applied knowledge on materials and processes with added value, and their transfer to the productive sectors at the local, national and European scales. We are involved in the training of new professionals on materials research, and in the dissemination of the scientific knowledge.

 

We embrace diversity, and we defend inclusive science as the only possible one.

 

How to arrive


Cursos Fronteras en Ciencia de Materiales

ICMM Congress

Forthcoming Events

APR15/12:00

Andreev bound states: detection and the road towards quantum technologies
Attila Geresdi  read more

APR22/12:00

Molecule-based magnetic heterostructures for spintronics and quantum computing
Enrique Burzurí  read more

Iron Oxide Nanoparticle Coatings Dictate Cell Outcomes Despite the Influence of Protein Coronas

Portilla Y., Mellid S., Paradela A., Ramos-Fernández A., Daviu N., Sanz-Ortega L., Pérez-Yagüe S., Morales M.P., Barber D.F.



A critical issue in nanomedicine is to understand the complex dynamics that dictate the interactions of nanoparticles (NPs) with their biological milieu. The most exposed part of a nanoparticle is its surface coating, which comes into contact with the biological medium and adsorbs proteins, forming what is known as a protein corona (PC). It is assumed that this PC mainly dictates the nanoparticle–cell interactions. As such, we set out to analyze how different coatings on iron oxide nanoparticles (MNPs) affect the composition of the PC that forms on top of them, and how these newly formed coronas influence the uptake of MNPs by macrophages and tumor cells, their subcellular location upon internalization, and their intracellular degradation. We found that different superficial charges of the coatings did not affect the PC composition, with an enrichment in proteins with affinity for divalent ions regardless of the type of coating. The iron oxide core of the MNP might become exposed to the biological medium, influencing the proteins that constitute the PCs. The presence of enzymes with hydrolase activity in the PC could explain the degradation of the coatings when they come into contact with the biological media. In terms of MNP internalization by cells, coatings mainly determine the endocytic pathways used, especially in terms of receptor-mediated endocytosis. However, the increase in hydrodynamic size provoked by the formation of the associated corona drives uptake mechanisms like macropinocytosis. Once inside the cells, the PC protected the NPs in their intracellular transit to lysosomes, where they were fully degraded. This understanding of how coatings and PCs influence different cellular processes will help design improved NPs for biomedical applications, taking into account the influence of the coating and corona on the biology of the NPs.

ACS Applied Materials and Interfaces

Comparison of the distribution of the “molecular function” of proteins identified in FBS and in the PC formed on DEX-MNPs. (a) Graph showing the percentage of proteins identified in FBS that belong to a certain “molecular function” group. (b) Graph showing the percentage of overexpressed proteins identified in the corona on DEX-MNPs that belong to a certain “molecular function” group. Different “molecular functions” are shown in different colors. The graphs in this figure were generated by the Panther Gene List software with an emphasis on binding and catalytic activity.

ICMM-2021 - Sor Juana Inés de la Cruz, 3, Cantoblanco, 28049 Madrid, Spain. Tel: +34 91 334 9000. info @ icmm.csic.es